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The report constructs containing 0. All constructs were confirmed by DNA sequencing. Cells were lysed in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitors Invitrogen. Protein concentrations were quantified by BCA assay Beyotime. Samples containing equal amounts of protein were electrophoresed as previously reported [ 2 ]. This program uses the median effect analysis algorithm, which produces the combination index CI value as a quantitative indicator of the degree of synergy or antagonism.
The injection was performed every weekly for three consecutive weeks. The animal weight, tumor volume, and feed intake were recorded. The tumor volume in mm 3 was calculated from the formula 0. At the end of the experiment, the whole blood was collected from mice orbit for liver function tests, and all tumors were resected for western blotting and immunohistochemistry. Kaplan—Meier and log-rank test were used for survival analysis.
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Drug combination studies and their synergy quantification using the Chou-Talalay method. J Neurosci. Download references. We would like to thank Professor Srikumar P. You can also search for this author in PubMed Google Scholar.
Correspondence to Huiqing Yuan. Reprints and Permissions. Wang, D. Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab.
Oncogene 38, — Download citation. Received : 25 June Revised : 24 November Accepted : 21 December Published : 15 January Issue Date : 02 May Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Communications Biology BMC Cancer Advanced search.
Skip to main content Thank you for visiting nature. Download PDF. Subjects Cancer therapeutic resistance Ubiquitylation. Abstract Anterior gradient 2 AGR2 , a protein belonging to the protein disulfide isomerase PDI family, is overexpressed in multiple cancers and promotes angiogenesis to drive cancer progression.
Introduction Human anterior gradient 2 AGR2 , a member of the protein disulfide isomerase PDI family, is firstly identified as differentially expressed in estrogen receptor-positive breast cancer cells [ 1 ]. Results Inhibition of proteasome decreases the AGR2 both at mRNA and protein levels AGR2 protein is highly expressed in various human cancers including lung cancer [ 12 ], which is associated with poor patient survival [ 4 ] and also confirmed in our analysis Fig.
Full size image. Discussion In the present study, we provided a novel explanation that proteasome inhibition resulted in a significant decrease of AGR2 in cancer cells. Transient transfection Cells were transfected with plasmids or siRNA oligonucleotides using Lipofectamine Invitrogen. Western blotting and immunoprecipitation Cells were lysed in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitors Invitrogen. Drug combination analysis CalcuSyn Biosoft, Ferguson, MO software was used for evaluation of interaction between drugs [ 45 ].
Statistical analysis Kaplan—Meier and log-rank test were used for survival analysis. References 1. Article Google Scholar 5. Article Google Scholar
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